product summary
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company name :
HUABIO
product type :
antibody
product name :
AQP1
catalog :
HA750378
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JM10-98
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of AQP1 on different lysates with Rabbit anti-AQP1 antibody (HA750378) at 1/2,000 dilution.
Lane 1: Human lung tissue lysate
Lane 2: Rat kidney tissue lysate
Lane 3: Rat kidney tissue lysate (no heat)
Lane 4: Rat lung tissue lysate
Lane 5: Rat lung tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28~40 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750378) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of AQP1 on different lysates with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse kidney tissue lysate (no heat)
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse lung tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28~40 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750378) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA750378
Target name :
AQP1
Species reactivity :
Human,Mouse,Rat,Cynomolgus monkey,Pig
Applications :
WB,IF-Tissue,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human AQP1 aa 245-269 / 269.
Uniprot id :
P29972>SwissProt: P29972 Human;SwissProt: Q02013 Mouse;SwissProt: P29975 Rat
Host :
Rabbit
Clone number :
JM10-98
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Human lung tissue lysate, rat kidney tissue lysate, rat lung tissue lysate, mouse kidney tissue lysate, mouse lung tissue lysate, MCF-7, Hela, SW480, human spleen tissue, human kidney tissue, human pancreas tissue, mouse kidney tissue, rat kidney tissue.
Molecular wt :
Predicted band size: 28 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000
;IF-Tissue: 1:500-1:2000
;IHC-P: 1:5,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA750378_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA750378_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA750378_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA750378_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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