EDG1 | HUABIO HA750376 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

EDG1

catalog :

HA750376

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM10-66

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (HA750376) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate Lane 3: bEnd.3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750376) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (HA750376) at 1/5,000 dilution. Lane 1: HepG2-si NT cell lysate Lane 2: HepG2-si EDG1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750376) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (HA750376) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: PC-12 cell lysate Lane 3: Rat brain tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20/40 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 60 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750376) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750376

Target name :

EDG1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human EDG1 aa 2-51 / 382.

Uniprot id :

P21453>SwissProt: P21453 Human;SwissProt: O08530 Mouse;SwissProt: P48303 Rat

Host :

Rabbit

Clone number :

JM10-66

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, HepG2 cell lysate, bEnd.3 cell lysate, HepG2, HUVEC, SH-SY5Y, human breast carcinoma tissue, human liver tissue, mouse brain tissue, mouse heart tissue.

Molecular wt :

Predicted band size: 43 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-5,000 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750376_4.jpg

Pic legend4 :

EDG1 was immunoprecipitated in 0.2mg HepG2 cell lysate with HA750376 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750376 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA750376 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA750376 in HepG2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 4 mintues

more info or order :

HUABIO product webpage