PGP9.5 | HUABIO HA750375 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PGP9.5

catalog :

HA750375

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM10-59

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PGP9.5 on different lysates with Rabbit anti-PGP9.5 antibody (HA750375) at 1/2,000 dilution. Lane 1: A-172 cell lysate (20 µg/Lane) Lane 2: SHG-44 cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: NCI-H1299 cell lysate (20 µg/Lane) Lane 6: A549 cell lysate (20 µg/Lane) Lane 7: 293T cell lysate (20 µg/Lane) Lane 8: Neuro-2a cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Lane 11: Mouse brain tissue lysate (30 µg/Lane) Lane 12: Rat brain tissue lysate (30 µg/Lane) Lane 13: LNCaP cell lysate (negative) (20 µg/Lane) Lane 14: K-562 cell lysate (negative) (20 µg/Lane) Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750375) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PGP9.5 on different lysates with Rabbit anti-PGP9.5 antibody (HA750375) at 1/5,000 dilution. Lane 1: A549-si NT cell lysate (10 µg/Lane) Lane 2: A549-si PGP9.5 cell lysate (10 µg/Lane) Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750375) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-PGP9.5 antibody (HA750375) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750375) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750375

Target name :

PGP9.5

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PGP95 aa 191-223 / 223.

Uniprot id :

P09936>SwissProt: P09936 Human;SwissProt: Q9R0P9 Mouse;SwissProt: Q00981 Rat

Host :

Rabbit

Clone number :

JM10-59

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A-172 cell lysate, SHG-44 cell lysate, U-87 MG cell lysate, SH-SY5Y cell lysate, NCI-H1299 cell lysate, A549 cell lysate, 293T cell lysate, Neuro-2a cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y, N2A, PC-12, mouse spinal cord tissue, mouse skin tissue, mouse brain tissue, mouse colon tissue, mouse cerebrum tissue.

Molecular wt :

Predicted band size: 25 kDa

Subcellular location :

Cytoplasm, Endoplasmic reticulum membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:1,000 ;IHC-P: 1:1,000-1:5,000 ;IP: Use at an assay dependent concentration. ;FC: 1:1,000 ;IHC-Fr: 1:200-1:500

Advanced Validation :

Relative expression (RE),Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750375_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PGP9.5 antibody (HA750375) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750375) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750375_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PGP9.5 antibody (HA750375) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750375) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750375_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-PGP9.5 antibody (HA750375) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750375) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750375_7.jpg

Pic legend7 :

Immunocytochemistry analysis of SH-SY5Y (positive) and LNCaP (negative) labeling PGP9.5 with Rabbit anti-PGP9.5 antibody (HA750375) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PGP9.5 antibody (HA750375) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750375_8.jpg

Pic legend8 :

Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 with Rabbit anti-PGP9.5 antibody (HA750375) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PGP9.5 antibody (HA750375) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750375_9.jpg

Pic legend9 :

Immunocytochemistry analysis of PC-12 cells labeling PGP9.5 with Rabbit anti-PGP9.5 antibody (HA750375) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PGP9.5 antibody (HA750375) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750375_10.jpg

Pic legend10 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750375_11.jpg

Pic legend11 :

Application: IHC-Fr Species: Mouse Site: Pancreas Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA750375_12.jpg

Pic legend12 :

Flow cytometric analysis of SH-SY5Y cells labeling PGP9.5. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750375, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage