Parvalbumin | HUABIO HA750371 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Parvalbumin

catalog :

HA750371

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM100-08

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Parvalbumin, HA750371, red); 1/500 (SLC32A1 / VGAT, HA601384, green) Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Parvalbumin, HA750371, red); 1/500 (SLC32A1 / VGAT, HA601384, green) Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

product information

SKU :

HA750371

Target name :

Parvalbumin

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Paravalbumin aa 1-110 / 110.

Uniprot id :

P20472>SwissProt: P20472 Human;SwissProt: P32848 Mouse;SwissProt: P02625 Rat

Host :

Rabbit

Clone number :

JM100-08

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse cerebellum tissue, rat cerebellum tissue, human cerebellum tissue, RPMI 8226 cell lysate, Mouse skeletal muscle tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate.

Molecular wt :

Predicted band size: 12 kDa

Subcellular location :

Axon, cytoplasm, synapse.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:5,000-1:50,000 ;IHC-Fr: 1:500-1:1,000 ;IF-Tissue: 1:500 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750371_4.jpg

Pic legend4 :

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750371_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750371) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750371_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750371) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750371_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750371) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750371_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750371) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750371_9.jpg

Pic legend9 :

Western blot analysis of Parvalbumin on different lysates with Rabbit anti-Parvalbumin antibody (HA750371) at 1/1,000 dilution. Lane 1: RPMI 8226 cell lysate (20 µg/Lane) Lane 2: SK-MEL-28 cell lysate (negative) (20 µg/Lane) Lane 3: Mouse skeletal muscle tissue lysate (20 µg/Lane) Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane) Lane 5: Rat cerebellum tissue lysate (40 µg/Lane) Predicted band size: 12 kDa Observed band size: 14 kDa Exposure time: Lane 1-3: 30 seconds; Lane 4-5: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750371_10.jpg

Pic legend10 :

Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750371_11.jpg

Pic legend11 :

Parvalbumin was immunoprecipitated from 0.2 mg RPMI 8226 cell lysate with HA750371 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750371 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RPMI 8226 cell lysate (input) Lane 2: HA750371 IP in RPMI 8226 cell lysate Lane 3: Rabbit IgG instead of HA750371 in RPMI 8226 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801

more info or order :

HUABIO product webpage