Complement C3 | HUABIO HA750367 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Complement C3

catalog :

HA750367

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF10-30

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Complement C3 on different lysates with Rabbit anti-Complement C3 antibody (HA750367) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Rat liver tissue lysate Lane 7: Rat spleen tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 187 kDa Observed band size: 108/37 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750367) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HepG2 cells labeling Complement C3 with Rabbit anti-Complement C3 antibody (HA750367) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Complement C3 antibody (HA750367) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Flow cytometric analysis of HepG2 cells labeling Complement C3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750367, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA750367

Target name :

Complement C3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human C3 aa 1,210-1,248 / 1,663.

Uniprot id :

P01024>SwissProt: P01024 Human;SwissProt: P01027 Mouse;SwissProt: P01026 Rat

Host :

Rabbit

Clone number :

JF10-30

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human liver tissue lysate, HepG2 cell lysate, HeLa cell lysate, Mouse liver tissue lysate, Mouse spleen tissue lysate, Rat liver tissue lysate, Rat spleen tissue lysate, HepG2.

Molecular wt :

Predicted band size: 187 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:1,000

more info or order :

HUABIO product webpage