Ctip2 | HUABIO HA750358 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ctip2

catalog :

HA750358

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF09-90

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex (P0) Sample: Frozen section Antibody concentration: 1:2,000 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex (P7) Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required

image 3 :

Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:200

product information

SKU :

HA750358

Target name :

Ctip2

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Ctip2 aa 491-534 / 894.

Uniprot id :

Q9C0K0>SwissProt: Q9C0K0 Human;SwissProt: Q99PV8 Mouse;Entrez Gene: 314423 Rat

Host :

Rabbit

Clone number :

JF09-90

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human brain tissue, mouse brain tissue, mouse cerebral cortex (P0) tissue, rat brain tissue, LO2 cell lysate, THP-1 cell lysate, Jurkat.

Molecular wt :

Predicted band size: 96 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:5,000-1:10,000 ;IHC-Fr: 1:1,000-1:2,000 ;IF-Tissue: 1:200-1:500 ;IF-Cell: 1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750358_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Ctip2 antibody (HA750358) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750358) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750358_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Ctip2 antibody (HA750358) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750358) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750358_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Ctip2 antibody (HA750358) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750358) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750358_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex (P0) tissue with Rabbit anti-Ctip2 antibody (HA750358) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750358) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750358_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Ctip2 antibody (HA750358) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750358) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750358_9.jpg

Pic legend9 :

Western blot analysis of Ctip2 on different lysates with Rabbit anti-Ctip2 antibody (HA750358) at 1/500 dilution. Lane 1: LO2 cell lysate Lane 2: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 96 kDa Observed band size: 130 kDa Exposure time: 3 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750358) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750358_10.jpg

Pic legend10 :

ICC staining of Ctip2 in LO2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750358, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

more info or order :

HUABIO product webpage