NF-H | HUABIO HA750357 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NF-H

catalog :

HA750357

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF99-07

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:2,000 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Mouse Site: Liver (Negative) Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

product information

SKU :

HA750357

Target name :

NF-H

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human NEFH aa 690-740.

Uniprot id :

P12036>SwissProt: P12036 Human;SwissProt: P19246 Mouse;SwissProt: P16884 Rat

Host :

Rabbit

Clone number :

JF99-07

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse brain tissue lysate, Rat brain tissue lysate, Rat hippocampus tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue.

Molecular wt :

Predicted band size: 200 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:1,000 ;IHC-Fr: 1:500-1:2,000 ;IF-Tissue: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750357_4.jpg

Pic legend4 :

Application: IHC-Fr Species: Rat Site: Liver (Negative) Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750357_5.jpg

Pic legend5 :

Western blot analysis of NF-H on different lysates with Rabbit anti-NF-H antibody (HA750357) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Rat brain tissue lysate (20 µg/Lane) Lane 3: Rat hippocampus tissue lysate (20 µg/Lane) Predicted band size: 200 kDa Observed band size: 250 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750357) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750357_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-NF-H antibody (HA750357) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750357) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750357_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-NF-H antibody (HA750357) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750357) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750357_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NF-H antibody (HA750357) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750357) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750357_9.jpg

Pic legend9 :

Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1:500

more info or order :

HUABIO product webpage