HMGCR | HUABIO HA750345 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HMGCR

catalog :

HA750345

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF0981

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of HMGCR on different lysates with Rabbit anti-HMGCR antibody (HA750345) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: THP-1 cell lysate Lane 3: HepG2 cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 98 kDa Observed band size: 100 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750345) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

HMGCR was immunoprecipitated in 0.2mg HeLa cell lysate with HA750345 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750345 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of HA750345 in HeLa cell lysate Lane 3: HA750345 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds

image 3 :

Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-HMGCR antibody (HA750345) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750345) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750345

Target name :

HMGCR

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human HMGCR aa 438-489 / 888.

Uniprot id :

P04035>SwissProt: P04035 Human;SwissProt: Q01237 Mouse;SwissProt: P51639 Rat

Host :

Rabbit

Clone number :

JF0981

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, THP-1 cell lysate, HepG2 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human small intestine tissue.

Molecular wt :

Predicted band size: 97 kDa

Subcellular location :

Endoplasmic reticulum membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IP: Use at an assay dependent concentration. ;IHC-P: 1:100

more info or order :

HUABIO product webpage