Myelin Basic Protein | HUABIO HA750337 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Myelin Basic Protein

catalog :

HA750337

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF0943

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

product information

SKU :

HA750337

Target name :

Myelin Basic Protein

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Myelin Basic Protein aa 121-304 / 304.

Uniprot id :

P02686>SwissProt: P02686 Human;SwissProt: P04370 Mouse;SwissProt: P02688 Rat

Host :

Rabbit

Clone number :

JF0943

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Rat brain tissue lysate, Mouse brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue, mouse cerebral cortex tissue, mouse hippocampus tissue.

Molecular wt :

Predicted band size: 33 kDa

Subcellular location :

Myelin membrane, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000-1:30,000 ;IF-Tissue: 1:500-1:1,000 ;IHC-Fr: 1:500-1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750337_4.jpg

Pic legend4 :

Application: IF-tissue Species: Human Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:500

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750337_5.jpg

Pic legend5 :

Application: IF-tissue Species: Mouse Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1:500

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750337_6.jpg

Pic legend6 :

Western blot analysis of Myelin Basic Protein on different lysates with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 14~25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750337) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750337_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750337) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750337_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue (negative) with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750337) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750337_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750337) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750337_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750337) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750337_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750337) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage