Actin | HUABIO HA750323 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Actin

catalog :

HA750323

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ09-29

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Actin on different lysates with Rabbit anti-Actin antibody (HA750323) at 1/10,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: A431 cell lysate (15 µg/Lane) Lane 3: NIH/3T3 cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (20 µg/Lane) Lane 6: Mouse colon tissue lysate (20 µg/Lane) Lane 7: Rat brain tissue lysate (20 µg/Lane) Lane 8: Rat colon tissue lysate (20 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750323) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IF-tissue Species: Human Site: Colon carcinoma Sample: Paraffin-embedded section Antibody concentration: 1/100

image 3 :

Western blot analysis of Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750323, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hybrid fish (crucian-carp) brain tissue lysate Lane 2: Hybrid fish (crucian-carp) kidney tissue lysate

product information

SKU :

HA750323

Target name :

Actin

Species reactivity :

Human,Mouse,Rat,Zebrafish

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Actin aa 45-80 / 377.

Uniprot id :

P68133>SwissProt: P68133 Human;SwissProt: P68134 Mouse;SwissProt: P68136 Rat;Entrez Gene: 407658 Zebrafish

Host :

Rabbit

Clone number :

JJ09-29

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, mouse colon tissue lysate, rat brain tissue lysate, rat colon tissue lysate, human colon carcinoma tissue, hybrid fish (crucian-carp) brain tissue lysate, hybrid fish (crucian-carp) kidney tissue lysate, NIH/3T3, mouse cardiac muscle tissue, mouse smooth muscle tissue.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Cytoplasm, Cytoskeleton.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:10,000-1:50,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:1,000 ;IHC-P: 1:5,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750323_4.jpg

Pic legend4 :

Immunocytochemistry analysis of NIH/3T3 cells labeling Actin with Rabbit anti-Actin antibody (HA750323) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Actin antibody (HA750323) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage