SOD2 | HUABIO HA750314 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SOD2

catalog :

HA750314

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ089-02

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SOD2 on different lysates with Rabbit anti-SOD2 antibody (HA750314) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: Human liver tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 25 kDa Observed band size: 23 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750314) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SOD2 antibody (HA750314) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750314) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SOD2 antibody (HA750314) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750314) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750314

Target name :

SOD2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human SOD2 aa 1-50 / 222.

Uniprot id :

P04179>SwissProt: P04179 Human;SwissProt: P09671 Mouse;SwissProt: P07895 Rat

Host :

Rabbit

Clone number :

JJ089-02

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, NIH/3T3 cell lysate, Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, mouse brain tissue, rat brain tissue, rat liver tissue.

Molecular wt :

Predicted band size: 25 kDa

Subcellular location :

Mitochondrion matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:1,000-1:8,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750314_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-SOD2 antibody (HA750314) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750314) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage