PD-L1 | HUABIO HA750309 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PD-L1

catalog :

HA750309

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ08-95

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (HA750309) at 1/5,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: A375 cell lysate Lane 3: U-87 MG cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 45 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750309) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer tissue using anti-PD-L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750309, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750309

Target name :

PD-L1

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PD-L1 aa 191-240 / 290.

Uniprot id :

Q9NZQ7>SwissProt: Q9NZQ7 Human

Host :

Rabbit

Clone number :

JJ08-95

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MDA-MB-231 cell lysate, A375 cell lysate, U-87 MG cell lysate, THP-1 cell lysate, human non-small cell lung cancer tissue.

Molecular wt :

Predicted band size: 33 kDa

Subcellular location :

Cell membrane, Early endosome membrane, Recycling endosome membrane, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:50-1:200

more info or order :

HUABIO product webpage