Phospho-AMPK alpha 1 (S496) | HUABIO HA750300 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-AMPK alpha 1 (S496)

catalog :

HA750300

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD0810

reactivity :

human, mouse

application :

western blot, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-AMPK alpha 1 (S496) on different lysates with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody (HA750300) at 1/5,000 dilution. Lane 1: SiHa cell lysate Lane 2: HUVEC cell lysate Lane 3: K-562 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750300) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-AMPK alpha 1(S496) on HUVEC cell lysates. Lane 1: HUVEC cells, whole cell lysate, 10ug/lane Lane 2/3: HUVEC cells treated with UV(2 hours recovery), whole cell lysates, 10ug/lane Lane 4: HUVEC cells treated with UV(2 hours recovery), then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-AMPK alpha 1(S496) antibody (HA750300) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size:64 kDa Observed band size:64 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 14 seconds

image 3 :

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Phospho-AMPK alpha 1 (S496) with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody (HA750300). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (HA750300, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

product information

SKU :

HA750300

Target name :

Phospho-AMPK alpha 1 (S496)

Species reactivity :

Human,Mouse

Applications :

WB,IP,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser496 of Human AMPK alpha 1 aa 471-520 / 559.

Uniprot id :

Q13131>SwissProt: Q13131 Human;SwissProt: Q5EG47 Mouse

Host :

Rabbit

Clone number :

SD0810

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SiHa cell lysate, HUVEC cell lysate, K-562 cell lysate, mouse hippocampus tissue, mouse cerebral cortex tissue.

Molecular wt :

Predicted band size: 64 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IP: Use at an assay dependent concentration. ;IHC-Fr: 1:100

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750300_4.jpg

Pic legend4 :

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Phospho-AMPK alpha 1 (S496) with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody (HA750300). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (HA750300, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

more info or order :

HUABIO product webpage