RUNX1+RUNX2+RUNX3 | HUABIO HA750296 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

RUNX1+RUNX2+RUNX3

catalog :

HA750296

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD0803

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of RUNX1+RUNX2+RUNX3 on different lysates with Rabbit anti-RUNX1+RUNX2+RUNX3 antibody (HA750296) at 1/1,000 dilution. Lane 1: Saos-2 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: SHG-44 cell lysate Lane 4: Mouse thymus tissue lysate Lysates/proteins at 10 µg/Lane1-3 and 20ug/Lane4. Predicted band size: 49 kDa Observed band size: 50 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750296) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of SHG-44 cells labeling RUNX1+RUNX2+RUNX3 with Rabbit anti-RUNX1+RUNX2+RUNX3 antibody (HA750296) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RUNX1+RUNX2+RUNX3 antibody (HA750296) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RUNX1+RUNX2+RUNX3 antibody. Counter stained with hematoxylin.

product information

SKU :

HA750296

Target name :

RUNX1+RUNX2+RUNX3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human RUNX1 aa 404-453 / 453.

Uniprot id :

Q01196>SwissProt: Q01196 Human;SwissProt: Q13761 Human;SwissProt: Q13950 Human;SwissProt: Q03347 Mouse;SwissProt: Q08775 Mouse;SwissProt: Q64131 Mouse;SwissProt: Q63046 Rat;SwissProt: Q9Z2J9 Rat

Host :

Rabbit

Clone number :

SD0803

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Saos-2 cell lysate, NIH/3T3 cell lysate, SHG-44 cell lysate, Mouse thymus tissue lysate, SHG-44, mouse testis tissue, human tonsil tissue.

Molecular wt :

49 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:1:1,000 ;IP: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750296_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX1+RUNX2+RUNX3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750296, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750296_5.jpg

Pic legend5 :

Flow cytometric analysis of SHG-44 cells labeling RUNX1+RUNX2+RUNX3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750296, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage