CD10 | HUABIO HA750273 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD10

catalog :

HA750273

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN75-07

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD10 on different lysates with Rabbit anti-CD10 antibody (HA750273) at 1/2,000 dilution. Lane 1: Ramos cell lysate (15 µg/Lane) Lane 2: Daudi cell lysate (15 µg/Lane) Lane 3: U-2 OS cell lysate (negative) (15 µg/Lane) Lane 4: Mouse kidney tissue lysate (20 µg/Lane) Lane 5: Mouse small intestine tissue lysate (20 µg/Lane) Lane 6: Rat lung tissue lysate (20 µg/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 24 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750273) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of 293 cells labeling CD10 with Rabbit anti-CD10 antibody (HA750273) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD10 antibody (HA750273) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling CD10 with Rabbit anti-CD10 antibody (HA750273) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750273, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

product information

SKU :

HA750273

Target name :

CD10

Species reactivity :

Human,Mouse,Rat

Applications :

WB,FC,IHC-P,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD10 aa 1-50 / 750.

Uniprot id :

P08473>SwissProt: P08473 Human;SwissProt: Q61391 Mouse;SwissProt: P07861 Rat

Host :

Rabbit

Clone number :

SN75-07

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Ramos cell lysate, Daudi cell lysate, Mouse kidney tissue lysate, Mouse small intestine tissue lysate, Rat lung tissue lysate, 293, human kidney tissue, human prostate carcinoma tissue, human small intestine tissue, human peripheral blood granulocytes.

Molecular wt :

Predicted band size: 86 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:50-1:200 ;FC: 1:1,000 ;IHC-P: 1:50-1:5,000 ;IF-Tissue: 1:100

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750273_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD10 antibody (HA750273) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750273) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750273_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-CD10 antibody (HA750273) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750273) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750273_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-CD10 antibody (HA750273) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750273) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750273_7.jpg

Pic legend7 :

Flow cytometric analysis of human peripheral blood granulocytes labeling CD10. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA750273, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage