Phospho-Tau (S396) | HUABIO HA750268 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-Tau (S396)

catalog :

HA750268

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN62-09

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-Tau (S396) on SHSY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750268, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-Tau(S396) on SHSY5Y cell lysates. Lane 1: SHSY5Y cells, whole cell lysate, 10ug/lane Lane 2: SHSY5Y cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Tau(S396) antibody (HA750268) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 79 kDa Observed band size: 70/130 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 34 seconds

image 3 :

ICC staining of Phospho-Tau (S396) in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750268, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

HA750268

Target name :

Phospho-Tau (S396)

Species reactivity :

Mouse,Rat,Human,Cynomolgus monkey,Pig

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser396 of human Tau.

Uniprot id :

P10636-8>SwissProt: P10636-8 Human;SwissProt: P10637 Mouse;SwissProt: P19332 Rat

Host :

Rabbit

Clone number :

SN62-09

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SHSY5Y cell lysates, N2A, PC-12, rat brain tissue, mouse brain tissue, mouse kidney tissue.

Molecular wt :

Predicted band size: 79 kDa

Subcellular location :

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:200 ;IHC-Fr:1:200-1:500

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750268_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-Tau (S396) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750268, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750268_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (S396) antibody (HA750268) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750268) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750268_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Tau (S396) antibody (HA750268) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750268) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750268_7.jpg

Pic legend7 :

Application: IHC-Fr Species: Mouse Site:Hippocampus Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750268_8.jpg

Pic legend8 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750268_9.jpg

Pic legend9 :

Western blot analysis of Phospho-Tau (S396) on different lysates with Rabbit anti-Phospho-Tau (S396) antibody (HA750268) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse brain tissue lysate treated with lambda-PP for 30 minutes Lysates/proteins at 40 µg/Lane. Predicted band size: 79 kDa Observed band size: 50-70, 130 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750268) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750268_10.jpg

Pic legend10 :

Immunocytochemistry analysis of PC-12 cells labeling Phospho-Tau (S396) with Rabbit anti-Phospho-Tau (S396) antibody (HA750268) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Tau (S396) antibody (HA750268) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage