CCR2 | HUABIO HA750266 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CCR2

catalog :

HA750266

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN707

reactivity :

human, mouse

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750266, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse spleen tissue lysate Lane 2: THP-1 cell lysate

image 2 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CCR2 antibody (HA750266) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750266) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CCR2 antibody (HA750266) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750266) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750266

Target name :

CCR2

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human CCR2.

Uniprot id :

P41597>SwissProt: P41597 Human;SwissProt: P51683 Mouse

Host :

Rabbit

Clone number :

SN707

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse spleen tissue lysate, THP-1 cell lysate, human tonsil tissue, human breast carcinoma tissue, human esophagus tissue, THP-1.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:500 ;FC: 1:500-1:1,000 ;IP: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750266_4.jpg

Pic legend4 :

Flow cytometric analysis of THP-1 cells labeling CCR2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA750266, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage