product summary
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company name :
HUABIO
product type :
antibody
product name :
HA tag
catalog :
HA750258
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SN07-06
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of HA tag on HA tag recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750258, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with HA750258 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/5,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 60 mins at room temperature.
Lane 1: HA Tag fusion protein lysate (input).
Lane 2: ET1611-49 IP in HA Tag fusion protein lysate.
Lane 3: 0906-1 IP in HA Tag fusion protein lysate.
Lane 4: Rabbit IgG instead of ET1611-49 in HA Tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST
product information
SKU :
HA750258
Target name :
HA tag
Species reactivity :
Species independent
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within N Terminal fusion protein and C Terminal fusion protein.
Uniprot id :
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Host :
Rabbit
Clone number :
SN07-06
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HA tag recombinant protein, C-terminal HA-tagged recombinant protein.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000
;IP: 2-5 µg/ml.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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