P Glycoprotein | HUABIO HA750249 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

P Glycoprotein

catalog :

HA750249

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN06-42

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of P Glycoprotein on different lysates with Rabbit anti-P Glycoprotein antibody (HA750249) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (no heat) (20 µg/Lane) Lane 2: HepG2 cell lysate Notice: no heat means the lysate is not boiled. Predicted band size: 141 kDa Observed band size: 150~250 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750249) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of P Glycoprotein on different lysates with Rabbit anti-P Glycoprotein antibody (HA750249) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (no heat) Lane 2: L-929 cell lysate (no heat) Lane 3: Mouse brain tissue lysate (no heat) Lane 4: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 141 kDa Observed band size: 150 kDa Exposure time: 1 minute 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750249) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of P Glycoprotein on different lysates with Rabbit anti-P Glycoprotein antibody (HA750249) at 1/1,000 dilution. Lane 1: Hela-si NT(no heat) cell lysate Lane 2: Hela-si P Glycoprotein(no heat) cell lysate Notice: no heat means the lysate is not boiled. Lysates/proteins at 10 µg/Lane. Predicted band size: 141 kDa Observed band size: 180 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. ET1611-30 was shown to specifically react with P Glycoprotein in Hela-si NT cells. Weakened band was observed when Hela-si P Glycoprotein sample was tested. Hela-si NT and Hela-si P Glycoprotein samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1611-30, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750249

Target name :

P Glycoprotein

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human P-glycoprotein 1 aa 373-730 / 1,280.

Uniprot id :

P08183>SwissProt: P08183 Human;SwissProt: P06795 Mouse;SwissProt: P43245 Rat

Host :

Rabbit

Clone number :

SN06-42

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, NIH/3T3 cell lysate, L-929 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human liver tissue, human kidney tissue, mouse brain tissue, rat brain tissue, C6, NIH/3T3.

Molecular wt :

Predicted band size: 141 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:200 ;IF-Tissue: 1:50 ;IF-Cell:1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750249_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750249, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750249_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-P Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750249, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750249_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-P Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750249, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750249_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750249, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750249_8.jpg

Pic legend8 :

Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling P Glycoprotein with Rabbit anti-P Glycoprotein antibody (HA750249) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750249, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750249_9.jpg

Pic legend9 :

Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling P Glycoprotein with Rabbit anti-P Glycoprotein antibody (HA750249) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750249, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750249_10.jpg

Pic legend10 :

Immunocytochemistry analysis of C6 cells labeling P Glycoprotein with Rabbit anti-P Glycoprotein antibody (HA750249) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-P Glycoprotein antibody (HA750249) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750249_11.jpg

Pic legend11 :

Immunocytochemistry analysis of NIH/3T3 cells labeling P Glycoprotein with Rabbit anti-P Glycoprotein antibody (HA750249) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-P Glycoprotein antibody (HA750249) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage