IKK alpha + IKK beta | HUABIO HA750248 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IKK alpha + IKK beta

catalog :

HA750248

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN63-02

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IKK alpha + IKK beta on different lysates with Rabbit anti-IKK alpha + IKK beta antibody (HA750248) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: Daudi cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 87 kDa Observed band size: 87 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750248) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of IKK alpha + IKK beta on different lysates with Rabbit anti-IKK alpha + IKK beta antibody (HA750248) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IKK beta KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 87 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750248) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of A431 cells labeling IKK alpha + IKK beta with Rabbit anti-IKK alpha + IKK beta antibody (HA750248) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IKK alpha + IKK beta antibody (HA750248) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750248

Target name :

IKK alpha + IKK beta

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human IKK beta aa 450-652 / 756.

Uniprot id :

O14920>SwissProt: O14920 Human;SwissProt: O15111 Human;SwissProt: O88351 Mouse;SwissProt: Q60680 Mouse;SwissProt: Q9QY78 Rat

Host :

Rabbit

Clone number :

SN63-02

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, 293T cell lysate, Daudi cell lysate, A431, NIH/3T3, C6.

Molecular wt :

Predicted band size: 87 kDa

Subcellular location :

Cytoplasm, Nucleus, Membrane raft.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100-1:500 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750248_4.jpg

Pic legend4 :

Immunocytochemistry analysis of NIH/3T3 cells labeling IKK alpha + IKK beta with Rabbit anti-IKK alpha + IKK beta antibody (HA750248) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IKK alpha + IKK beta antibody (HA750248) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750248_5.jpg

Pic legend5 :

Immunocytochemistry analysis of C6 cells labeling IKK alpha + IKK beta with Rabbit anti-IKK alpha + IKK beta antibody (HA750248) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IKK alpha + IKK beta antibody (HA750248) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage