Western blot analysis of Active+Pro Caspase-9 on different lysates with Rabbit anti-Active+Pro Caspase-9 antibody (HA750238) at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750238) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Caspase-9 on different lysates with Rabbit anti-Caspase-9 antibody (HA750238) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si Caspase-9 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. ET1610-95 was shown to specifically react with Caspase-9 in Hela-si NT cells. Weakened band was observed when Hela-si Caspase-9 sample was tested. Hela-si NT and Hela-si Caspase-9 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-95, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.