CD14 | HUABIO HA750232 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD14

catalog :

HA750232

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC69-02

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD14 on different lysates with Rabbit anti-CD14 antibody (HA750232) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 40 kDa Observed band size: 60 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750232) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CD14 on different lysates with Rabbit anti-CD14 antibody (HA750232) at 1/1,000 dilution. Lane 1: SW480-si NT cell lysate Lane 2: SW480-si CD14 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 50-60 kDa Exposure time: 7 seconds; ECL: merk 4-20% SDS-PAGE gel. ET1610-85 was shown to specifically react with CD14 in SW480-si NT cells. Weakened band was observed when SW480-si CD14 sample was tested. SW480-si NT and SW480-si CD14 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-85, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of THP-1 cells labeling CD14 with Rabbit anti-CD14 antibody (HA750232) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD14 antibody (HA750232) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750232

Target name :

CD14

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD14 aa 310-335 / 375.

Uniprot id :

P08571>SwissProt: P08571 Human;SwissProt: P10810 Mouse

Host :

Rabbit

Clone number :

SC69-02

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysates, A549, NCCIT, NIH/3T3, LO2, human tonsil tissue, human liver tissue, human colon carcinoma tissue, human spleen tissue, human uterus tissue, human lymph nodes tissue, human cervical cancer.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

Cell membrane, Secreted, Golgi apparatus, Membrane raft.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:200-1:800 ;FC: 1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750232_4.jpg

Pic legend4 :

Immunocytochemistry analysis of NIH/3T3 cells labeling CD14 with Rabbit anti-CD14 antibody (HA750232) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD14 antibody (HA750232) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750232_5.jpg

Pic legend5 :

Flow cytometric analysis of human peripheral blood cells labelling CD14 (HA750232).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750232_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD14 antibody (HA750232) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750232) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750232_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD14 antibody (HA750232) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750232) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750232_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD14 antibody (HA750232) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750232) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage