Mast Cell Tryptase | HUABIO HA750229 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Mast Cell Tryptase

catalog :

HA750229

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC68-07

reactivity :

human, mouse

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Mast Cell Tryptase on different lysates with Rabbit anti-Mast Cell Tryptase antibody (HA750229) at 1/1,000 dilution. Lane 1: Human lung tissue lysate (40 µg/Lane) Lane 2: Mouse skin tissue lysate (40 µg/Lane) Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750229) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Mast Cell Tryptase antibody (HA750229) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750229) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750229

Target name :

Mast Cell Tryptase

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Mast Cell Tryptase aa 10-275 / 275.

Uniprot id :

Q15661>SwissProt: Q15661 Human;SwissProt: Q02844 Mouse

Host :

Rabbit

Clone number :

SC68-07

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human lung tissue lysates, mouse skin tissue lysates, human lung tissue, human tonsil tissue, human small intestine tissue, human prostate carcinoma tissue.

Molecular wt :

Predicted band size: 30 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:5,000-1:10,000 ;IP: Use at an assay dependent concentration.

more info or order :

HUABIO product webpage