Tryptophan Hydroxylase 1 (TPH1) | HUABIO HA750214 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Tryptophan Hydroxylase 1 (TPH1)

catalog :

HA750214

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC53-07

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Tryptophan Hydroxylase 1 (TPH1) on different lysates with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (HA750214) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: LNCaP cell lysate Lane 3: HL-60 cell lysate Lane 4: THP-1 cell lysate Lane 5: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 72 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750214) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of TPH1 on different lysates with Rabbit anti-TPH1 antibody (HA750214) at 1/1,000 dilution. Lane 1: MDA-MB-231-si NT cell lysate (10 µg/Lane) Lane 2: MDA-MB-231-si TPH1 cell lysate (10 µg/Lane) Predicted band size: 51 kDa Observed band size: 72 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. ET1610-37 was shown to specifically react with TPH1 in MDA-MB-231-si NT cells. Weakened band was observed when MDA-MB-231-si TPH1 sample was tested. MDA-MB-231-si NT and Hela-si TPH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-37, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (HA750214) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750214) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750214

Target name :

Tryptophan Hydroxylase 1 (TPH1)

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human TPH1 aa 372-419 / 444.

Uniprot id :

P17752>SwissProt: P17752 Human;SwissProt: P17532 Mouse;SwissProt: P09810 Rat

Host :

Rabbit

Clone number :

SC53-07

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MDA-MB-231 cell lysate, LNCaP cell lysate, HL-60 cell lysate, THP-1 cell lysate, MCF7 cell lysate, human brain tissue, rat brain tissue, mouse cerebellum tissue, Hela.

Molecular wt :

Predicted band size: 51 kDa

Subcellular location :

Cytosol, neuron projection.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750214_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Tryptophan Hydroxylase 1 (TPH1) antibody (HA750214) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750214) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750214_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Tryptophan Hydroxylase 1 (TPH1) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750214, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750214_6.jpg

Pic legend6 :

Flow cytometric analysis of Tryptophan Hydroxylase 1 (TPH1) was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750214, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage