Phospho-Tau (T231) | HUABIO HA750210 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-Tau (T231)

catalog :

HA750210

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC58-08

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-Tau (T231) on different lysates with Rabbit anti-Phospho-Tau (T231) antibody (HA750210) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain treated with λpp for 1 hour tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750210) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA750210) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750210) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA750210) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750210) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750210

Target name :

Phospho-Tau (T231)

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IP,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr231 of human Tau-F (P10636-8).

Uniprot id :

P10636-8>SwissProt: P10636-8 Human;SwissProt: P10637 Mouse;SwissProt: P19332 Rat

Host :

Rabbit

Clone number :

SC58-08

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue, rat brain tissue, human brain tissue, mouse hippocampus tissue.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Secreted, cytoskeleton, cell membrane, cytosol, axon, dendrite.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200 ;IHC-Fr: 1:200-1:500

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750210_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA750210) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750210) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750210_5.jpg

Pic legend5 :

Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1/200

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750210_6.jpg

Pic legend6 :

Application: IF-tissue Species: Mouse Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1/200

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750210_7.jpg

Pic legend7 :

Application: IF-tissue Species: Rat Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1/200

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750210_8.jpg

Pic legend8 :

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750210_9.jpg

Pic legend9 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

more info or order :

HUABIO product webpage