Aurora B | HUABIO HA750208 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Aurora B

catalog :

HA750208

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC55-08

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Aurora B on different lysates using anti-Aurora B antibody at 1/1,000 dilution. Positive control: Lane 1: Hela cell lysates Lane 2: MCF-7 cell lysates

image 2 :

All lanes: Western blot analysis of Aurora B with anti-Aurora B antibody (HA750208) at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2: Aurora B knockdown Hela whole cell lysate (10 µg). ET1610-25 was shown to specifically react with Aurora B in wild-type Hela cells. Weakened band was observed when Aurora B knockdown sample was tested. Wild-type and Aurora B knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-25, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Aurora B antibody. Counter stained with hematoxylin.

product information

SKU :

HA750208

Target name :

Aurora B

Species reactivity :

Human

Applications :

WB,IHC-P,IP,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Aurora B aa 1-50 / 344.

Uniprot id :

Q96GD4>SwissProt: Q96GD4 Human;SwissProt: O70126 Mouse;SwissProt: O55099 Rat

Host :

Rabbit

Clone number :

SC55-08

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Hela cell lysate, MCF-7 cell lysate, human tonsil tissue, HeLa.

Molecular wt :

Predicted band size: 39 kDa

Subcellular location :

Nucleus, Cytoplasm, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750208_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HeLa cells labeling Aurora B with Rabbit anti-Aurora B antibody (HA750208) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aurora B antibody (HA750208) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

more info or order :

HUABIO product webpage