TOMM20 | HUABIO HA750166 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TOMM20

catalog :

HA750166

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST04-72

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TOMM20 on different lysates with Rabbit anti-TOMM20 antibody (HA750166) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: Saos-2 cell lysate (low expression) (15 µg/Lane) Lane 3: HepG2 cell lysate (15 µg/Lane) Lane 4: A549 cell lysate (15 µg/Lane) Lane 5: NIH/3T3 cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Lane 7: C6 cell lysate (15 µg/Lane) Lane 8: PC-12 cell lysate (15 µg/Lane) Lane 9: Mouse brain tissue lysate (30 µg/Lane) Lane 10: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750166) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of TOMM20 on different lysates with Rabbit anti-TOMM20 antibody (HA750166) at 1/5,000 dilution. Lane 1: HeLa-si NT cell lysate (10 µg/Lane) Lane 2: HeLa-si TOMM20 cell lysate (10 µg/Lane) Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750166) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of TOMM20 on different lysates with Rabbit anti-TOMM20 antibody (HA750166) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: Saos-2 cell lysate (low expression) (10 µg/Lane) Lane 3: HepG2 cell lysate (10 µg/Lane) Lane 4: A549 cell lysate (10 µg/Lane) Lane 5: MCF7 cell lysate (10 µg/Lane) Lane 6: NIH/3T3 cell lysate (10 µg/Lane) Lane 7: F9 cell lysate (10 µg/Lane) Lane 8: PC-12 cell lysate (10 µg/Lane) Lane 9: Mouse brain tissue lysate (20 µg/Lane) Lane 10: Rat brain tissue lysate (20 µg/Lane) Lane 11: Rat lung tissue lysate (20 µg/Lane) Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 1 minute 22 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750166) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750166

Target name :

TOMM20

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue,IF-Cell,FC,IP,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human TOMM20 aa 1-145 / 145.

Uniprot id :

Q15388>SwissProt: Q15388 Human;SwissProt: Q9DCC8 Mouse;SwissProt: Q62760 Rat

Host :

Rabbit

Clone number :

ST04-72

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, Saos-2 cell lysate, HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, MCF7 cell lysate, F9 cell lysate, Rat lung tissue lysate, HepG2, NIH/3T3, HeLa, human kidney tissue, rat kidney tissue, mouse kidney tissue, human liver tissue, rat large intestine tissue.

Molecular wt :

Predicted band size: 16 kDa

Subcellular location :

Mitochondrion outer membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:20,000 ;IHC-P: 1:500-1:1,000 ;IHC-Fr: 1:500 ;IF-Tissue: 1:500 ;IF-Cell: 1:1,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Relative expression (RE),Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750166_4.jpg

Pic legend4 :

Application: IF-cell Species: Human Cell Sample: HeLa cell Antibody concentration: 1: 1,000 Date by conrtesy of: Mr. Wenxiang Huang School of Basic Medical Sicences, Zhejiang University

Pic img5 :

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Pic legend5 :

Application: IF-cell Species: Human Cell Sample: AC16 cell Antibody concentration: 1: 200 Date by conrtesy of: Mr. Zhiyi Yang School of Basic Medical Sicences, Zhejiang University

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750166_6.jpg

Pic legend6 :

Immunocytochemistry analysis of HepG2 (high expression) and Saos-2 (low expression) labeling TOMM20 with Rabbit anti-TOMM20 antibody (HA750166) at 1/1,000 dilution and competitor's antibody at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TOMM20 antibody (HA750166) at 1/1,000 dilution and competitor's antibody at 1/400 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750166_7.jpg

Pic legend7 :

Immunocytochemistry analysis of NIH/3T3 cells labeling TOMM20 with Rabbit anti-TOMM20 antibody (HA750166) at 1/1,000 dilution and competitor's antibody at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TOMM20 antibody (HA750166) at 1/1,000 dilution and competitor's antibody at 1/400 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750166_8.jpg

Pic legend8 :

Immunocytochemistry analysis of HeLa cells labeling TOMM20 with Rabbit anti-TOMM20 antibody (HA750166) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TOMM20 antibody (HA750166) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750166_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TOMM20 antibody (HA750166) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750166) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750166_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TOMM20 antibody (HA750166) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750166) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750166_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TOMM20 antibody (HA750166) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750166) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA750166_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-TOMM20 antibody (HA750166) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750166) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA750166_13.jpg

Pic legend13 :

Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-TOMM20 antibody (HA750166) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750166) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img14 :

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Pic legend14 :

Application: IHC-Fr Species: Mouse Site: Kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Pic img15 :

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Pic legend15 :

Application: IHC-Fr Species: Rat Site: Kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Pic img16 :

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Pic legend16 :

TOMM20 was immunoprecipitated in 0.2mg HeLa cell lysate with HA750166 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750166 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of HA750166 in HeLa cell lysate Lane 3: HA750166 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 2 seconds

Pic img17 :

https://storage.huabio.cn/huabio/productImg/HA750166_17.jpg

Pic legend17 :

Flow cytometric analysis of HeLa cells labeling TOMM20. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750166, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage