Calreticulin | HUABIO HA750156 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Calreticulin

catalog :

HA750156

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SU37-03

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Calreticulin on different lysates with Rabbit anti-Calreticulin antibody (HA750156) at 1/50,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: HL-60 cell lysate Lane 4: C2C12 cell lysate Lane 5: C6 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Rat liver tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750156) at 1/50,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Calreticulin on different lysates with Rabbit anti-Calreticulin antibody (HA750156) at 1/50,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-Calreticulin KD cell lysate (10 µg/Lane) Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750156) at 1/50,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of Calreticulin on different lysates with Rabbit anti-Calreticulin antibody (HA750156) at 1/50,000 dilution. Lane 1: HeLa-si NT cell lysate (10 µg/Lane) Lane 2: HeLa-si Calreticulin cell lysate (10 µg/Lane) Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 13 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750156) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750156

Target name :

Calreticulin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Calreticulin aa 40-89 / 417.

Uniprot id :

P27797>SwissProt: P27797 Human;SwissProt: P14211 Mouse;SwissProt: P18418 Rat

Host :

Rabbit

Clone number :

SU37-03

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, HeLa cell lysate, HL-60 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, human liver carcinoma tissue, HeLa.

Molecular wt :

Predicted band size: 48 kDa

Subcellular location :

Endoplasmic reticulum lumen, Cytoplasm, Secreted, Cell surface, Sarcoplasmic reticulum lumen, nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:50,000 ;IF-Cell: 1:2,000 ;IHC-P: 1:5,000 ;FC: 1:2,000 ;IP: 1-2μg/sample

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750156_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Calreticulin antibody (HA750156) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750156) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750156_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Calreticulin antibody (HA750156) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750156) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750156_6.jpg

Pic legend6 :

Immunocytochemistry analysis of HeLa cells labeling Calreticulin with Rabbit anti-Calreticulin antibody (HA750156) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Calreticulin antibody (HA750156) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750156_7.jpg

Pic legend7 :

Flow cytometric analysis of HeLa cells labeling Calreticulin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750156, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750156_8.jpg

Pic legend8 :

Calreticulin was immunoprecipitated in 0.2mg HeLa cell lysate with HA750156 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750156 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of HA750156 in HeLa cell lysate Lane 3: HA750156 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds

more info or order :

HUABIO product webpage