CD31 | HUABIO HA750151 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD31

catalog :

HA750151

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SU03-59

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD31 on THP-1 cell lysates with Rabbit anti-CD31 antibody (HA750151) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750151) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

CD31 was immunoprecipitated in 0.2mg THP-1 cell lysate with HA750151 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750151 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input) Lane 2: Rabbit IgG instead of HA750151 in THP-1 cell lysate Lane 3: HA750151 IP in THP-1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD31 antibody (HA750151) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750151) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750151

Target name :

CD31

Species reactivity :

Human

Applications :

WB,IHC-P,FC,IP,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD31 aa 689-738 / 738.

Uniprot id :

P16284>SwissProt: P16284 Human

Host :

Rabbit

Clone number :

SU03-59

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 cell lysate, human tonsil tissue, THP-1.

Molecular wt :

Predicted band size: 83 kDa

Subcellular location :

Cell junction. Cell membrane. Membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P:1:50-1:1,000 ;FC: 1:50-1:100 ;IP: 1-2μg/sample ;IF-Cell: 1:100 ;IF-Tissue: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750151_4.jpg

Pic legend4 :

Flow cytometric analysis of CD31 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750151, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750151_5.jpg

Pic legend5 :

Immunocytochemistry analysis of THP-1 cells labeling CD31 with Rabbit anti-CD31 antibody (HA750151) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD31 antibody (HA750151) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750151_6.jpg

Pic legend6 :

Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling CD31 with Rabbit anti-CD31 antibody (HA750151) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750151, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage