Phospho-IRF3 (S386) | HUABIO HA750138 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-IRF3 (S386)

catalog :

HA750138

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SU03-28

reactivity :

human

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-IRF3 (S386) on different lysates with Rabbit anti-Phospho-IRF3 (S386) antibody (HA750138) at 1/1,000 dilution. Lane 1: MCF7 whole cell lysate Lane 2: MCF7 treated with 100nM Calyculin A for 30 minutes whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750138) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining of Phospho-IRF3 (S386) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750138, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

HA750138

Target name :

Phospho-IRF3 (S386)

Species reactivity :

Human

Applications :

WB,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser386 of human IRF3.

Uniprot id :

Q14653>SwissProt: Q14653 Human

Host :

Rabbit

Clone number :

SU03-28

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 treated with 100nM Calyculin A for 30 minutes whole cell lysate, MCF-7.

Molecular wt :

Predicted band size: 47 kDa

Subcellular location :

Cytoplasm, Nucleus, Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage