NADPH oxidase 4/NOX4 | HUABIO HA750122 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NADPH oxidase 4/NOX4

catalog :

HA750122

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SY0214

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NADPH oxidase 4/NOX4 on different lysates with Rabbit anti-NADPH oxidase 4/NOX4 antibody (HA750122) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: U-2 OS cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750122) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of NOX4 with anti-NOX4 antibody [SY0214] (HA750122) at 1/1,000 dilution. Lane 1: Wild-type SH-SY5Y whole cell lysate (20 µg). Lane 2/3: NOX4 fragment 1 knockdown SH-SY5Y whole cell lysate (20 µg). Lane 4/5: NOX4 fragment 2 knockdown SH-SY5Y whole cell lysate (20 µg). Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1607-4, 1/1,000) and Loading control antibody(Rabbit anti-HSP90, ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.

image 3 :

NADPH oxidase 4/NOX4 was immunoprecipitated in 0.2mg SH-SY5Y cell lysate with HA750122 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750122 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y cell lysate (input) Lane 2: Rabbit IgG instead of HA750122 in SH-SY5Y cell lysate Lane 3: HA750122 IP in SH-SY5Y cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL: K1801

product information

SKU :

HA750122

Target name :

NADPH oxidase 4/NOX4

Species reactivity :

Human,Rat,Mouse

Applications :

WB,IF-Cell,IHC-P,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human NOX4 aa 460-578 / 578.

Uniprot id :

Q9NPH5>SwissProt: Q9NPH5 Human;SwissProt: Q924V1 Rat

Host :

Rabbit

Clone number :

SY0214

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, U-2 OS cell lysate, SH-SY5Y, human lung tissue, human kidney tissue, rat stomach tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 67 kDa

Subcellular location :

Cell membrane, Nucleus, Endoplasmic reticulum membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:200-1:500 ;IP: 1-2μg/sample ;FC: 1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750122_4.jpg

Pic legend4 :

Immunocytochemistry analysis of SH-SY5Y cells labeling NADPH oxidase 4/NOX4 with Rabbit anti-NADPH oxidase 4/NOX4 antibody (HA750122) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NADPH oxidase 4/NOX4 antibody (HA750122) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750122_5.jpg

Pic legend5 :

Flow cytometric analysis of SH-SY5Y cells labeling NADPH oxidase 4/NOX4. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750122, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750122_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NADPH oxidase 4/NOX4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750122, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750122_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NADPH oxidase 4/NOX4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750122, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage