product summary
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company name :
HUABIO
product type :
antibody
product name :
N Cadherin
catalog :
HA750119
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SY02-46
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
image
image 1 :
Western blot analysis of N Cadherin on different lysates with Rabbit anti-N Cadherin antibody (HA750119) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: A549 cell lysate
Lane 3: HeLa cell lysate
Lane 4: A-172 cell lysate
Lane 5: MCF7 cell lysate (negative)
Lane 6: C2C12 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 140-150 kDa
Exposure time: 2 minutes 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750119) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of N Cadherin on different lysates with Rabbit anti-N Cadherin antibody (HA750119) at 1/5,000 dilution.
Lane 1: 293T-si NT cell lysate (10 µg/Lane)
Lane 2: 293T-si N Cadherin cell lysate (10 µg/Lane)
Predicted band size: 100 kDa
Observed band size: 150 kDa
Exposure time: 1 minute 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750119) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Application: IHC-Fr
Species: Mouse
Site: Liver
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
product information
SKU :
HA750119
Target name :
N Cadherin
Species reactivity :
Human,Mouse,Rat,Cynomolgus monkey,Pig
Applications :
WB,IHC-P,IHC-Fr,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human N Cadherin aa 161-210 / 906.
Uniprot id :
P19022>SwissProt: P19022 Human;SwissProt: P15116 Mouse;SwissProt: Q9Z1Y3 Rat
Host :
Rabbit
Clone number :
SY02-46
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
293T cell lysate, A549 cell lysate, HeLa cell lysate, A-172 cell lysate, MCF7 cell lysate, C2C12 cell lysate, C6 cell lysate, mouse liver tissue, rat liver tissue, human liver carcinoma tissue, human liver tissue, mouse heart tissue, Hela.
Molecular wt :
Predicted band size: 100 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000-1:20,000
;IHC-P: 1:10,000-1:40,000
;IHC-Fr: 1:500-1:1,000
;IF-Tissue: 1:2,000
Advanced Validation :
Relative expression (RE),Knockdown (KD)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA750119_4.jpg
Pic legend4 :
Application: IHC-Fr
Species: Mouse
Site: Liver
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA750119_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-N Cadherin antibody (HA750119) at 1/40,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750119) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA750119_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-N Cadherin antibody (HA750119) at 1/40,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750119) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA750119_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-N Cadherin antibody (HA750119) at 1/40,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750119) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA750119_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-N Cadherin antibody (HA750119) at 1/40,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750119) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/HA750119_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-N Cadherin antibody (HA750119) at 1/40,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750119) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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