GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with HA750116 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/10,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature. Lane 1: GFP Tag fusion protein lysate (input). Lane 2: Rabbit IgG instead of ET1607-31 in GFP Tag fusion protein lysate. Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate. Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate. Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate. Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate. Lane 7: R1312-2 IP in GFP Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST

Immunocytochemistry analysis of HeLa cells transfected with N-terminal GFP labeling GFP with Rabbit anti-GFP antibody (HA750116) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GFP antibody (HA750116) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with GFP (green). Nuclear DNA was labelled in blue with DAPI.