Notch 1 | HUABIO HA750111 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Notch 1

catalog :

HA750111

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SJ205

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Notch 1 on different lysates with Rabbit anti-Notch 1 antibody (HA750111) at 1/5,000 dilution. Lane 1: C6 cell lysate (20 µg/Lane) Lane 2: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 273 kDa Observed band size: 125/273 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750111) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Notch 1 on different lysates with Rabbit anti-Notch 1 antibody (HA750111) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: THP-1 cell lysate Lane 3: A549 cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 273 kDa Observed band size: 125/273 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750111) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of Notch 1 on different lysates with Rabbit anti-Notch 1 antibody (HA750111) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si Notch 1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 273 kDa Observed band size: 125 kDa Exposure time: 6 minutes; 4-20% SDS-PAGE gel. ET1606-55 was shown to specifically react with Notch 1 in Hela-si NT cells. Weakened band was observed when Hela-si Notch 1 sample was tested. Hela-si NT and Hela-si Notch 1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-55, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750111

Target name :

Notch 1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Notch 1 aa 2,481-2,530 / 2,555.

Uniprot id :

P46531>SwissProt: P46531 Human;SwissProt: Q01705 Mouse;SwissProt: Q07008 Rat

Host :

Rabbit

Clone number :

SJ205

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

C6 cell lysate, rat brain tissue lysate, HeLa cell lysate, THP-1 cell lysate, A549 cell lysate, HEK-293 cell lysate, Hela, HepG2, NIH/3T3, human pancreas tissue, mouse lung tissue, mouse pancreas tissue.

Molecular wt :

Predicted band size: 273 kDa

Subcellular location :

Cell membrane, Late endosome membrane; Nucleus (intracellular domain).

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750111_4.jpg

Pic legend4 :

ICC staining of Notch 1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750111, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750111_5.jpg

Pic legend5 :

ICC staining of Notch 1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750111, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750111_6.jpg

Pic legend6 :

ICC staining of Notch 1 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750111, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750111_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Notch 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750111, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750111_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Notch 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750111, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750111_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Notch 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750111, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750111_10.jpg

Pic legend10 :

Immunofluorescence analysis of paraffin-embedded human liver cancer tissue labeling Notch 1 with Rabbit anti-Notch 1 antibody (HA750111) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750111, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750111_11.jpg

Pic legend11 :

Flow cytometric analysis of Notch 1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750111, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

more info or order :

HUABIO product webpage