VEGF Receptor 1 | HUABIO HA750081 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

VEGF Receptor 1

catalog :

HA750081

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SY09-09

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of VEGF Receptor 1 on different lysates with Rabbit anti-VEGF Receptor 1 antibody (HA750081) at 1/5,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: Human brain tissue lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 151 kDa Observed band size: 151 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750081) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of VEGF Receptor 1 on different lysates with Rabbit anti-VEGF Receptor 1 antibody (HA750081) at 1/1,000 dilution. Lane 1: MCF7-si NT cell lysate (10 µg/Lane) Lane 2: MCF7-si VEGF Receptor 1 cell lysate (10 µg/Lane) Predicted band size: 151 kDa Observed band size: 151 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. ET1605-11 was shown to specifically react with VEGF Receptor 1 in Hela-si NT cells. Weakened band was observed when Hela-si VEGF Receptor 1 sample was tested. Hela-si NT and Hela-si VEGF Receptor 1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1605-11, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

VEGF Receptor 1 was immunoprecipitated in 0.2mg MCF7 cell lysate with HA750081 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750081 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input) Lane 2: Rabbit IgG instead of HA750081 in MCF7 cell lysate Lane 3: HA750081 IP in MCF7 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds

product information

SKU :

HA750081

Target name :

VEGF Receptor 1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human VEGF Receptor 1 aa 1-50 / 1,338.

Uniprot id :

P17948>SwissProt: P17948 Human;SwissProt: P35969 Mouse;SwissProt: P53767 Rat

Host :

Rabbit

Clone number :

SY09-09

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, N2A, RH-35, SHG-44, mouse placenta tissue, mouse brain tissue, A431.

Molecular wt :

Predicted band size: 151 kDa

Subcellular location :

Cell membrane, Endosome, Secreted, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:50-1:100 ;IF-Tissue: 1:50-1:100 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750081_4.jpg

Pic legend4 :

ICC staining of VEGF Receptor 1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750081, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750081_5.jpg

Pic legend5 :

ICC staining of VEGF Receptor 1 in RH-35 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750081, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750081_6.jpg

Pic legend6 :

ICC staining of VEGF Receptor 1 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750081, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750081_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-VEGF Receptor 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750081, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750081_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VEGF Receptor 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750081, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750081_9.jpg

Pic legend9 :

Flow cytometric analysis of VEGF Receptor 1 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750081, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage