Heme Oxygenase 1 (HO-1) | HUABIO HA750079 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Heme Oxygenase 1 (HO-1)

catalog :

HA750079

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SP08-07

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Flow cytometric analysis of Jurkat cells labeling Heme Oxygenase 1 (HO-1). Cells were fixed and permeabilized. Then stained with the primary antibody (HA750079, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 2 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (HA750079) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750079) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (HA750079) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750079) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750079

Target name :

Heme Oxygenase 1 (HO-1)

Species reactivity :

Human

Applications :

WB,IHC-P,IP,FC,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human HO-1 aa 227-276 / 288.

Uniprot id :

P09601>SwissProt: P09601 Human

Host :

Rabbit

Clone number :

SP08-07

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human spleen tissue, human liver tissue, Jurkat.

Molecular wt :

Predicted band size: 33 kDa

Subcellular location :

Endoplasmic reticulum membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:50-1:1,000 ;FC: 1:500-1:1,000 ;IP: Use at an assay dependent concentration. ;IF-Tissue: 1:50-1:200

more info or order :

HUABIO product webpage