Bcl-2 | HUABIO HA750057 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Bcl-2

catalog :

HA750057

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SZ10-03

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Bcl-2 on different lysates with Rabbit anti-Bcl-2 antibody (HA750057) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: HL-60 cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750057) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Bcl-2 on different lysates with Rabbit anti-Bcl-2 antibody (HA750057) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si Bcl-2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 31 seconds; ECL: merk 4-20% SDS-PAGE gel. ET1603-11 was shown to specifically react with Bcl-2 in Hela-si NT cells. Weakened band was observed when Hela-si Bcl-2 sample was tested. Hela-si NT and Hela-si Bcl-2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1603-11, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining of Bcl-2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750057, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

HA750057

Target name :

Bcl-2

Species reactivity :

Human

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Bcl-2 aa 40-90.

Uniprot id :

P10415>SwissProt: P10415 Human

Host :

Rabbit

Clone number :

SZ10-03

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, Jurkat cell lysate, MCF7 cell lysate, HL-60 cell lysate, THP-1 cell lysate, A549, MCF-7, SH-SY5Y, human lymph nodes tissue, human kidney tissue, Jurkat.

Molecular wt :

Predicted band size: 26 kDa

Subcellular location :

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:800 ;IHC-P: 1:50-1:4,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750057_4.jpg

Pic legend4 :

ICC staining of Bcl-2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750057, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750057_5.jpg

Pic legend5 :

ICC staining of Bcl-2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750057, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750057_6.jpg

Pic legend6 :

Flow cytometric analysis of Bcl-2 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750057, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750057_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Bcl-2 antibody (HA750057) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750057) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750057_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-Bcl-2 antibody (HA750057) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750057) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage