PUMA | HUABIO HA750038 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PUMA

catalog :

HA750038

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SR42-09

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PUMA on different lysates with Rabbit anti-PUMA antibody (HA750038) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HDLM-2 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750038) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PUMA on different lysates with Rabbit anti-PUMA antibody (HA750038) at 1/1,000 dilution. Lane 1: Rat stomach tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750038) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining of PUMA in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750038, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

HA750038

Target name :

PUMA

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PUMA aa 153-193 / 193.

Uniprot id :

Q9BXH1>SwissProt: Q9BXH1 Human;SwissProt: Q99ML1 Mouse;SwissProt: Q80ZG6 Rat

Host :

Rabbit

Clone number :

SR42-09

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HepG2 cell lysate, HDLM-2 cell lysate, NIH/3T3 cell lysate, Rat stomach tissue lysate, Rat liver tissue lysate, Hela, SKOV-3, human stomach cancer tissue, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue, rat stomach tissue.

Molecular wt :

Predicted band size: 21 kDa

Subcellular location :

Mitochondrion

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50 ;IF-Tissue: 1:200-1:500 ;IHC-P: 1:2,000-1:30,000 ;FC: 1:50

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750038_4.jpg

Pic legend4 :

ICC staining of PUMA in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750038, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750038_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-PUMA antibody (HA750038) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750038) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750038_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PUMA antibody (HA750038) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750038) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750038_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-PUMA antibody (HA750038) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750038) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750038_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-PUMA antibody (HA750038) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750038) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750038_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-PUMA antibody (HA750038) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750038) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750038_10.jpg

Pic legend10 :

Flow cytometric analysis of PUMA was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750038, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

more info or order :

HUABIO product webpage