Collagen XVII | HUABIO HA750030 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Collagen XVII

catalog :

HA750030

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SR46-05

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody (HA750030) at 1/5,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: Mouse skin tissue lysate (20 µg/Lane) Lane 3: Rat skin tissue lysate (20 µg/Lane) Predicted band size: 148 kDa Observed band size: 130、180 kDa Exposure time: 40 seconds; ECL: K1802;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750030) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody (HA750030) at 1/1,000 dilution. Lane 1: Wild-type caski whole cell lysate (10 µg). Lane 2/3: Collagen XVII knockdown caski whole cell lysate (10 µg). Predicted band size: 148 kDa Observed band size: 130、180 kDa Exposure time: 1 minutes 40 seconds; ECL: K1801;4-20% SDS-PAGE gel. HA750030 was shown to specifically react with Collagen XVII in wild-type caski cells. Weakened bands were observed when Collagen XVII knockdown samples were tested. Wild-type and Collagen XVII knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA750030) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen XVII antibody (HA750030) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750030) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750030

Target name :

Collagen XVII

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Tissue,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Collagen XVII aa 1313-1420 / 1497.

Uniprot id :

Q9UMD9>SwissProt: Q9UMD9 Human;SwissProt: Q07563 Mouse;Unigene:40392 Rat

Host :

Rabbit

Clone number :

SR46-05

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, mouse skin tissue lysate, rat skin tissue lysate, HUVEC, human skin tissue, mouse skin tissue, rat skin tissue.

Molecular wt :

Predicted band size: 148 kDa

Subcellular location :

Cell junction, Membrane, Secreted

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Tissue: 1:50 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750030_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Collagen XVII antibody (HA750030) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750030) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750030_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Collagen XVII antibody (HA750030) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750030) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage