product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
CD9
catalog :
HA750027
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SA35-08
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (HA750027) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: HepG2 cell lysate
Lane 6: SK-MEL-28 cell lysate
Lane 7: A375 cell lysate
Lane 8: B16-F1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 23 kDa
Exposure time: 3 minutes 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750027) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
ICC staining of CD9 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750027, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
image 3 :
ICC staining of CD9 in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750027, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
product information
SKU :
HA750027
Target name :
CD9
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P,IP,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human CD9 aa 179-228 / 228.
Uniprot id :
P21926>SwissProt: P21926 Human;SwissProt: P40240 Mouse;SwissProt: P40241 Rat
Host :
Rabbit
Clone number :
SA35-08
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, HCT 116 cell lysate, HepG2 cell lysate, SK-MEL-28 cell lysate, A375 cell lysate, B16-F1 cell lysate, SW480, CRC, human tonsil tissue, human spleen tissue, human kidney tissue, mouse brain tissue, mouse spleen tissue.
Molecular wt :
Predicted band size: 25 kDa
Subcellular location :
Cell membrane, Membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
;IF-Cell: 1:50
;IHC-P: 1:200-1:1,000
;IP: 1-2μg/sample
;FC:1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA750027_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750027, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA750027_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750027, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA750027_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD9 antibody (HA750027) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA750027_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CD9 antibody (HA750027) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA750027_8.jpg
Pic legend8 :
Flow cytometric analysis of B16-F1 cells labeling CD9.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA750027, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img9 :
https://storage.huabio.cn/huabio/productImg/HA750027_9.jpg
Pic legend9 :
CD9 was immunoprecipitated from 0.2 mg K-562 cell lysate with HA750027 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750027 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate (input)
Lane 2: HA750027 IP in K-562 cell lysate
Lane 3: Rabbit IgG instead of HA750027 in K-562 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 11 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
related products
browse more products
questions and comments