GAPDH | HUABIO HA750022 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GAPDH

catalog :

HA750022

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA30-01

reactivity :

human, mouse, rat, chicken, zebrafish , fruit fly

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GAPDH on Hela cell lysates with Rabbit anti-GAPDH antibody (HA750022). Hela cell lysates at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750022) at serial dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750022, 1/100,000) was used in 5% NFDM/TBST at room temperature for 1 hour. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 45 mins at room temperature. Positive control: Lane 1: Rat liver tissue lysate, 20 µg/Lane Lane 2: Rat lung tissue lysate, 20 µg/Lane Lane 3: Rat lung tissue lysate, 20 µg/Lane Lane 4: Rat heart tissue lysate, 20 µg/Lane Lane 5: Rat cerebellum tissue lysate, 20 µg/Lane Lane 6: Rat skeletal muscle tissue lysate, 20 µg/Lane Lane 7: Rat spleen tissue lysate, 20 µg/Lane Lane 8: Rat small intestine tissue lysate, 20 µg/Lane

image 3 :

Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (HA750022) at 1/80,000 dilution. Cell lysates at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750022) at 1/80,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750022

Target name :

GAPDH

Species reactivity :

Human,Mouse,Rat,Chicken,Drosophila melanogaster,Zebrafish

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse GAPDH aa 94-333 / 333.

Uniprot id :

P04406>SwissProt: P04406 Human;SwissProt: P16858 Mouse;SwissProt: P04797 Rat

Host :

Rabbit

Clone number :

SA30-01

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Rat liver tissue lysate, rat lung tissue lysate, rat heart tissue lysate, rat cerebellum tissue lysate, rat skeletal muscle tissue lysate, rat spleen tissue lysate, rat small intestine tissue lysate, hybrid fish (crucian-carp) brain tissue lysates, PC-3 cell lysate, mouse colon tissue lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, SK-Br-3 cell lysate, rat brain tissue lysate, A549, HepG2, human liver tissue, mouse liver tissue, mouse spleen tissue, DF-1 cell lysate.

Molecular wt :

Predicted band size: 36 kDa

Subcellular location :

Cytoplasm, cytosol, Nucleus perinuclear region, Membrane, cytoskeleton.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:50,000-1:200,000 ;IF-Cell: 1:1,000 ;IF-Tissue: 1:200-1:500 ;IHC-P: 1:1,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750022_4.jpg

Pic legend4 :

Western blot analysis of GAPDH on DF-1 cell lysates with Rabbit anti-GAPDH antibody (HA750022) at 1/100,000 dilution. Cell lysates at 15 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 second; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 2 hour at room temperature. The primary antibody (HA750022) at 1/100,000 dilution was used in 5% NFDM/TBST at 4 ℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 2 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750022_5.jpg

Pic legend5 :

Western blot analysis of GAPDH on zebrafish tissue lysates with Rabbit anti-GAPDH antibody (HA750022) at 1/50,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750022) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750022_6.jpg

Pic legend6 :

Western blot analysis of GAPDH on fruit flies tissue lysates with Rabbit anti-GAPDH antibody (HA750022) at 1/100,000 dilution. Lysates/proteins at 8 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750022) at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750022_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GAPDH antibody (HA750022) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750022) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750022_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-GAPDH antibody (HA750022) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750022) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750022_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-GAPDH antibody (HA750022) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750022) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750022_10.jpg

Pic legend10 :

Immunocytochemistry analysis of HeLa cells labeling GAPDH with Rabbit anti-GAPDH antibody (HA750022) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GAPDH antibody (HA750022) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750022_11.jpg

Pic legend11 :

Immunocytochemistry analysis of NIH/3T3 cells labeling GAPDH with Rabbit anti-GAPDH antibody (HA750022) at 1/2,500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GAPDH antibody (HA750022) at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA750022_12.jpg

Pic legend12 :

Flow cytometric analysis of HeLa cells labeling GAPDH. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750022, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA750022_13.jpg

Pic legend13 :

GAPDH was immunoprecipitated from 0.2 mg A549 cell lysate with HA750022 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750022 at 1/20,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: HA750022 IP in A549 cell lysate Lane 3: Rabbit IgG instead of HA750022 in A549 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 7 seconds; ECL: K1801

more info or order :

HUABIO product webpage