p75 NGF Receptor | HUABIO HA750014 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

p75 NGF Receptor

catalog :

HA750014

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA39-02

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of p75 NGF Receptor on Neuro-2a cell lysates with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 45 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of p75 NGF Receptor on PC-12 cell lysates with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 45 kDa Observed band size: 70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750014) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of p75 NGF Receptor on different lysates with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/2,000 dilution. Lane 1: PC-12-si NT cell lysate Lane 2: PC-12-si p75 NGF Receptor cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 70 kDa Exposure time: 2 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750014

Target name :

p75 NGF Receptor

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human p75 NGF Receptor aa 345-394 / 427.

Uniprot id :

P08138>SwissProt: P08138 Human;SwissProt: Q9Z0W1 Mouse;SwissProt: P07174 Rat

Host :

Rabbit

Clone number :

SA39-02

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Neuro-2a cell lysates, PC-12 cell lysates, Hela, rat uterus tissue, human endometrium tissue, human tonsil tissue, mouse uterus tissue, SH-SY5Y cell lysates, Neuro-2a, PC-12.

Molecular wt :

Predicted band size: 45 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:50-1:100 ;IF-Tissue: 1:50-1:100 ;IHC-P: 1:200-1:1,000 ;IP: 1-2μg/sample ;FC: 1:200 ;IHC-Fr: 1:500-1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750014_4.jpg

Pic legend4 :

Immunocytochemistry analysis of Hela cells labeling p75 NGF Receptor with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750014_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat uterus tissue with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750014) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750014_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750014) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750014_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750014) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750014_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750014, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750014_9.png

Pic legend9 :

Flow cytometric analysis of PC-12 cells labeling p75 NGF Receptor. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750014, 1:200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750014_10.jpg

Pic legend10 :

Western blot analysis of p75 NGF Receptor on SH-SY5Y cell lysates with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/2,000 dilution. Lysates/proteins at 20µg/Lane. Predicted band size: 45 kDa Observed band size: 70 kDa Exposure time: 1 minutes 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750014_11.jpg

Pic legend11 :

Immunocytochemistry analysis of Neuro-2a cells labeling p75 NGF Receptor with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA750014_12.jpg

Pic legend12 :

Immunocytochemistry analysis of PC-12 cells labeling p75 NGF Receptor with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA750014_13.jpg

Pic legend13 :

p75 NGF Receptor was immunoprecipitated from 0.2 mg C6 cell lysate with HA750014 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750014 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: C6 cell lysate (input) Lane 2: HA750014 IP in C6 cell lysate Lane 3: Rabbit IgG instead of HA750014 in C6 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 20 seconds; ECL: K1801

more info or order :

HUABIO product webpage