p53 | HUABIO HA750005 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

p53

catalog :

HA750005

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA39-07

reactivity :

human

application :

western blot, chromatin immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (HA750005) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: Saos-2 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750005) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (HA750005) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-p53 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750005) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of 293T cells labeling p53 with Rabbit anti-p53 antibody (HA750005) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (HA750005) at 1/400 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750005

Target name :

p53

Species reactivity :

Human

Applications :

WB,IF-Cell,ChIP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human p53 aa 1-100.

Uniprot id :

P04637>SwissProt: P04637 Human

Host :

Rabbit

Clone number :

SA39-07

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T cell lysate, 293T.

Molecular wt :

Predicted band size: 53 kDa

Subcellular location :

Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:400 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Advanced Validation :

Relative expression (RE),Knockdown (KD),Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750005_4.jpg

Pic legend4 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 treated with UV for 40minutes then recover for 3 hours cells with p53 (HA750005) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage