antibody

SP1

HA750$330

100μl

649.00 USD

monoclonal

domestic rabbit

nonconjugated

JF0950

human, mouse, rat

western blot, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section

SP1

Human,Mouse,Rat

WB,IF-Cell,IF-Tissue,IHC-P,FC,ChIP,IP

Non-conjugated

Synthetic peptide within Human SP1 aa 544-588 / 785.

P08047>SwissProt: P08047 Human;SwissProt: O89090 Mouse;SwissProt: Q01714 Rat

Rabbit

JF0950

IgG

100μl

649.00 USD

PBS (pH7.4).

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, U-2 OS cell lysate, COS-1 cell lysate, HeLa, human breast cancer tissue, human colon cancer tissue, human lung tissue, mouse lung tissue, rat lung tissue, MCF-7.

Predicted band size: 81 kDa

Nucleus, Cytoplasm.

1 mg/mL.

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:200-1:1,000 ;FC: 1:500-1:1,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin. ;IP: 1-2μg/sample

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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SP1 antibody (HA750$330) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750$330) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-SP1 antibody (HA750$330) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750$330) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SP1 antibody (HA750$330) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750$330) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-SP1 antibody (HA750$330) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750$330) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Flow cytometric analysis of MCF-7 cells labeling SP1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750$330, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with SP1 (HA750$330) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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SP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA750$330 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750$330 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA750$330 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA750$330 in HeLa cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 6 seconds; ECL: K1801