product summary
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company name :
HUABIO
product type :
antibody
product name :
Complement C3
catalog :
HA723984
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH18-31
reactivity :
mouse, rat
application :
western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA723984 image 1
Western blot analysis of Complement C3 on different lysates with Rabbit anti-Complement C3 antibody (HA723984) at 1/2,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat liver tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 186 kDa Observed band size: 43-186 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723984) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA723984 image 2
Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
image 3 :
HUABIO HA723984 image 3
Application: IHC-Fr Species: Rat Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
product information
SKU :
HA723984
Target name :
Complement C3
Species reactivity :
Mouse,Rat
Applications :
WB,IHC-Fr,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within mouse Complement C3 aa 1-1,663.
Uniprot id :
P01027>SwissProt: P01027 Mouse;SwissProt: P01026 Rat
Host :
Rabbit
Clone number :
PSH18-31
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Mouse liver tissue lysate, Mouse spleen tissue lysate, Rat liver tissue lysate, Rat spleen tissue lysate, mouse liver tissue.
Molecular wt :
Predicted band size: 186 kDa
Subcellular location :
Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000 ;IHC-Fr: 1:500 ;IHC-P: 1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA723984_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Complement C3 antibody (HA723984) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723984) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!