IGFBP1 | HUABIO HA723981 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IGFBP1

catalog :

HA723981

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH18-18

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IGFBP1 on different lysates with Rabbit anti-IGFBP1 antibody (HA723981) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: SK-MEL-28 cell lysate (negative) (30 µg/Lane) Lane 3: MDA-MB-231 cell lysate (30 µg/Lane) Predicted band size: 28 kDa Observed band size: 34 kDa Exposure time: Lane 1: 20 seconds; Lane 2-3: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723981) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HepG2 (positive) and SK-MEL-28 (negative) labeling IGFBP1 with Rabbit anti-IGFBP1 antibody (HA723981) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IGFBP1 antibody (HA723981) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Flow cytometric analysis of SK-MEL-28 (left, negative) and HepG2 (right, positive) cells labeling IGFBP1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723981, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA723981

Target name :

IGFBP1

Species reactivity :

Human

Applications :

WB,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human 1-259.

Uniprot id :

P08833>SwissProt: P08833 Human

Host :

Rabbit

Clone number :

PSH18-18

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, MDA-MB-231 cell lysate, HepG2.

Molecular wt :

Predicted band size: 28 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723981_4.jpg

Pic legend4 :

IGFBP1 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA723981 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723981 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA723981 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA723981 in HepG2 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 20 seconds; ECL: K1801

more info or order :

HUABIO product webpage