product summary
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company name :
HUABIO
product type :
antibody
product name :
heavy chain Myosin / MYH3
catalog :
HA723975
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH18-12
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of heavy chain Myosin / MYH3 on different lysates with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
Lane 1: RD cell lysate (20 µg/Lane)
Lane 2: Mouse embryo tissue lysate (20 µg/Lane)
Lane 3: Mouse testis tissue lysate (negative) (20 µg/Lane)
Lane 4: Rat embryo tissue lysate (20 µg/Lane)
Lane 5: Rat testis tissue lysate (negative) (20 µg/Lane)
Predicted band size: 224 kDa
Observed band size: 250 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723975) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/30,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA723975
Target name :
heavy chain Myosin / MYH3
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within mouse MYH3 aa 1,811-1,860.
Uniprot id :
P11055>SwissProt: P11055 Human;SwissProt: P13541 Mouse;SwissProt: P12847 Rat
Host :
Rabbit
Clone number :
PSH18-12
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
RD cell lysate, Mouse embryo tissue lysate, Rat embryo tissue lysate, human fetal skeletal muscle tissue, mouse E14.5 embryo tissue, rat E14.5 embryo tissue.
Molecular wt :
Predicted band size: 224 kDa
Subcellular location :
Cytoplasm, myofibril.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000
;IHC-P: 1:5,000-1:30,000
;IP: 1-2μg/sample
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA723975_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse adult skeletal muscle tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA723975_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse testis tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA723975_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded rat E14.5 embryo tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA723975_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat adult skeletal muscle tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA723975_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded rat testis tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/HA723975_9.jpg
Pic legend9 :
heavy chain Myosin / MYH3 was immunoprecipitated from 0.2 mg RD cell lysate with HA723975 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723975 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: RD cell lysate (input)
Lane 2: HA723975 IP in RD cell lysate
Lane 3: Rabbit IgG instead of HA723975 in RD cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 20 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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