MURF1 | HUABIO HA723973 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MURF1

catalog :

HA723973

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH18-10

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MURF1 on different lysates with Rabbit anti-MURF1 antibody (HA723973) at 1/5,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) (20 µg/Lane) Lane 3: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (negative) (40 µg/Lane) Lane 5: Mouse heart tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time:1 minute 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723973) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of MURF1 on different lysates with Rabbit anti-MURF1 antibody (HA723973) at 1/5,000 dilution. Lane 1: Rat skeletal muscle tissue lysate (40 µg/Lane) Lane 2: Rat liver tissue lysate (negative) (40 µg/Lane) Lane 3: Rat heart tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723973) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Flow cytometric analysis of SK-MEL-28 cells labeling MURF1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723973, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA723973

Target name :

MURF1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MURF1 aa 180-343 /353

Uniprot id :

Q969Q1>SwissProt: Q969Q1 Human;SwissProt: Q38HM4 Mouse;SwissProt: Q91Z63 Rat

Host :

Rabbit

Clone number :

PSH18-10

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SK-MEL-28 cell lysate, Mouse skeletal muscle tissue lysate, Mouse heart tissue lysate, Rat skeletal muscle tissue lysate, Rat heart tissue lysate, SK-MEL-28.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

Cytoplasm, Nucleus, myofibril, sarcomere, M line, Z line.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723973_4.jpg

Pic legend4 :

MURF1 was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with HA723973 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723973 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SK-MEL-28 cell lysate (input) Lane 2: HA723973 IP in SK-MEL-28 cell lysate Lane 3: Rabbit IgG instead of HA723973 in SK-MEL-28 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801

more info or order :

HUABIO product webpage