antibody

TPP1

HA723972

100μl

385.00 USD

monoclonal

domestic rabbit

nonconjugated

PSH18-09

human, mouse, rat

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

TPP1

Human,Mouse,Rat

WB,IHC-P,IF-Cell,FC,IP

Non-conjugated

Recombinant protein within human TPP1 aa 1-563.

O14773>SwissProt: O14773 Human;SwissProt: O89023 Mouse;SwissProt: Q9EQV6 Rat

Rabbit

PSH18-09

IgG

100μl

385.00 USD

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

HepG2 cell lysate, HeLa cell lysate, Jurkat cell lysate, Raji cell lysate, 786-0 cell lysate, A431 cell lysate, JAR cell lysate, U-87 MG cell lysate, Mouse heart tissue lysate, Mouse cerebellum tissue lysate, Mouse skeletal muscle tissue lysate, Rat heart tissue lysate, Rat cerebellum tissue lysate, Rat skeletal muscle tissue lysate, human skeletal muscle tissue, human cerebellum tissue, human placenta tissue, mouse cerebellum tissue, mouse placenta tissue, rat cerebellum tissue, rat placenta tissue, Raji, HepG2.

Predicted band size: 61 kDa

Lysosome, Melanosome.

1 mg/mL.

WB:1:5,000 ;IHC-P:1:1,000 ;IF-Cell:1:100-1:200 ;FC:1:1,000 ;IP: 1-2μg/sample

https://storage.huabio.cn/huabio/productImg/HA723972_4.jpg

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-TPP1 antibody (HA723972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA723972_5.jpg

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-TPP1 antibody (HA723972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA723972_6.jpg

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-TPP1 antibody (HA723972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA723972_7.jpg

Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-TPP1 antibody (HA723972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA723972_8.jpg

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-TPP1 antibody (HA723972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA723972_9.jpg

Immunohistochemical analysis of paraffin-embedded rat placenta tissue with Rabbit anti-TPP1 antibody (HA723972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA723972_10.jpg

Immunocytochemistry analysis of Raji cells labeling TPP1 with Rabbit anti-TPP1 antibody (HA723972) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TPP1 antibody (HA723972) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

https://storage.huabio.cn/huabio/productImg/HA723972_11.jpg

Immunocytochemistry analysis of HepG2 cells labeling TPP1 with Rabbit anti-TPP1 antibody (HA723972) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TPP1 antibody (HA723972) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

https://storage.huabio.cn/huabio/productImg/HA723972_12.jpg

Flow cytometric analysis of Raji cells labeling TPP1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723972, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

https://storage.huabio.cn/huabio/productImg/HA723972_13.jpg

Flow cytometric analysis of HepG2 cells labeling TPP1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723972, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

https://storage.huabio.cn/huabio/productImg/HA723972_14.jpg

TPP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723972 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723972 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723972 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723972 in HeLa cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 6 seconds; ECL: K1801