PADI2 | HUABIO HA723970 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PADI2

catalog :

HA723970

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH18-07

reactivity :

human

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PADI2 on different lysates with Rabbit anti-PADI2 antibody (HA723970) at 1/5,000 dilution. Lane 1: PANC-1 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: MCF7 cell lysate (low expression) Lane 4: HT-29 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 76 kDa Observed band size: 75 kDa Exposure time: Lane 1-2: 6 seconds; Lane 3-5: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723970) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

PADI2 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with HA723970 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723970 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PANC-1 cell lysate (input) Lane 2: HA723970 IP in PANC-1 cell lysate Lane 3: Rabbit IgG instead of HA723970 in PANC-1 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 seconds; ECL: K1801

product information

SKU :

HA723970

Target name :

PADI2

Species reactivity :

Human,Monkey

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human PADI2 aa 1-350.

Uniprot id :

Q9Y2J8>SwissProt: Q9Y2J8 Human

Host :

Rabbit

Clone number :

PSH18-07

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

PANC-1 cell lysate, SK-Br-3 cell lysate, MCF7 cell lysate, HT-29 cell lysate, COS-1 cell lysate.

Molecular wt :

Predicted band size: 76 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage